Journal: Cancer Cell International

Article Title: The addition of arginine deiminase potentiates Mithramycin A-induced cell death in patient-derived glioblastoma cells via ATF4 and cytochrome C

doi: 10.1186/s12935-023-02873-2

Figure Lengend Snippet: Effects of MitA on cellular radiosensitivity. A GBM cells were treated with increasing concentrations [2–100 nM] for 24 h before irradiation with 2 Gy (IBL637) and subsequent medium change [light purple] vs . non-irradiated MitA-treated cells [dark purple] for 2 × 72 h. Quantitative analyses were done using Calcein-AM assay. n = 3–7 independent experiments. *p < 0.05, **p < 0.01; ***p < 0.001; ****p < 0.0001 vs. control, Two-way ANOVA (Tukey’s multiple comparison test). B Representative images of 2D-cultured GBM cells (HROG02, HROG05, HROG63) stained with anti-H2A.X Phospho (Ser139) [red], treated with 5 nM MitA or left untreated with [ +] and without [−] irradiation. Scale bar as indicated: 50 µm. Nuclei were counterstained with DAPI. Images were taken on a Zeiss Elyra 7 Confocal Laser Microscope. Representative images of n = 3 independent experiments

Article Snippet: Images were taken on a Zeiss Elyra 7 Confocal Laser Microscope.

Techniques: Irradiation, Calcein AM Assay, Cell Culture, Staining, Microscopy

Journal: Cancer Cell International

Article Title: The addition of arginine deiminase potentiates Mithramycin A-induced cell death in patient-derived glioblastoma cells via ATF4 and cytochrome C

doi: 10.1186/s12935-023-02873-2

Figure Lengend Snippet: Cytotoxic effects of MitA and SpyADI combination. A Bliss independence calculation after combination treatment of GBM cell lines HROG02, HROG05, HROG52, and HROG63. Synergistic effects were obtained in 3/4 cell lines by applying the SIM combination approach. B γ-H2AX staining [red] of HROG05 and HROG63 treated with SpyADI and SIM combination with [ +] and without [−] radiation. Scale bar as indicated: 50 µm. Nuclei were counterstained with DAPI. Images were taken on a Zeiss Elyra 7 Confocal Laser Microscope. Representative images of n = 3 independent experiments. C HROG cells received 10 × 72 h consecutive mono- and SIM combination therapy cycles and stained with crystal violet to identify long-term treatment effects. Viability reduction (%) after treatment (HROG05: dark purple; HROG63: light purple) was quantified by normalization to control values (untreated cells, set to be 100%). n = 3 independent experiments. *p < 0.05, **p < 0.01; ***p < 0.001; ****p < 0.0001 vs. control; #### p < 0.0001 vs. monotherapy; $$$$ p < 0.0001 vs. SEQ-combination, Two-way ANOVA (Tukey's multiple comparisons test). D Viability in the 3D model in mono- and SIM combination regimens (HROG05 [black], HROG63 [grey]) was assessed with CellTiter-Glo 3D. n = 3 independent experiments. **p < 0.01; ****p < 0.0001 vs. control; ##p < 0.01 vs. monotherapy, Two-way ANOVA (Tukey's multiple comparisons test)

Article Snippet: Images were taken on a Zeiss Elyra 7 Confocal Laser Microscope.

Techniques: Staining, Microscopy

Journal: Cancer Cell International

Article Title: The addition of arginine deiminase potentiates Mithramycin A-induced cell death in patient-derived glioblastoma cells via ATF4 and cytochrome C

doi: 10.1186/s12935-023-02873-2

Figure Lengend Snippet: Pluripotency and self-renewal of HROG05 cells. A , B Cells were treated, fixed, permeabilized and stained with Phalloidin green, Alexa Fluor 594 GFAP [red], Alexa Fluor 647 Oct4 [purple], Alexa Flour 488 Nanog [green] and counterstained with DAPI. Images were taken on a Zeiss Elyra 7 Confocal Laser Microscope. A Representative single channel and merged images are shown. B Quantitative analysis of specific markers in relation to the control (= 100%). Mean + SEM, n = 3 independent experiments

Article Snippet: Images were taken on a Zeiss Elyra 7 Confocal Laser Microscope.

Techniques: Staining, Microscopy